Raab-Graham K F, Radeke C M, Vandenberg C A
Department of Biological Sciences, University of California, Santa Barbara 93106.
Neuroreport. 1994 Dec 20;5(18):2501-5. doi: 10.1097/00001756-199412000-00024.
We have isolated a cDNA encoding an inwardly rectifying K+ channel (HH-IRK1) from human heart. The cDNA codes for a 427-amino acid protein, with two putative transmembrane domains and an H5 region. The primary structure of HH-IRK1 is homologous to that of the IRK1 channel cloned from a mouse macrophage-like cell line. Functional expression in Xenopus oocytes showed that HH-IRK1 is a K+ channel with strong inward rectification, blocked by extracellular Ba2+ and Cs+, and with a single-channel conductance of 30 pS. Northern analysis showed HH-IRK1 transcripts in human heart, brain, skeletal muscle, placenta, lung and kidney. HH-IRK1 was mapped to human chromosome 17.
