Genome Research Center and Division of Allergy and Respiratory Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, Republic of Korea.
Department of Interdisciplinary Program in Biomedical Science Major, Soonchunhyang Graduate School, Bucheon, Republic of Korea.
Can Respir J. 2020 Feb 25;2020:3406530. doi: 10.1155/2020/3406530. eCollection 2020.
Fibroblast dysfunction is the main pathogenic mechanism underpinning idiopathic pulmonary fibrosis (IPF). Potassium voltage-gated channel subfamily J member 2 (KCNJ2) plays critical roles in the proliferation of myofibroblasts and in the development of cardiac fibrosis.
This study aimed to evaluate the role of KCNJ2 in IPF.
mRNA expression was measured using real-time PCR in fibroblasts from IPF patients and normal controls (NCs). Protein concentrations were measured by ELISA in bronchoalveolar lavage (BAL) fluid obtained from NCs ( = 30), IPF ( = 30), IPF ( = 30), IPF ( = 30), IPF ( = 30), IPF (.
mRNA expression was measured using real-time PCR in fibroblasts from IPF patients and normal controls (NCs). Protein concentrations were measured by ELISA in bronchoalveolar lavage (BAL) fluid obtained from NCs ( = 30), IPF ( = 30), IPF ( < 0.001). KCNJ2 protein levels in BAL fluid were significantly higher in IPF (6.587 [1.441-26.01] ng/mL) than in NCs (0.084 [0.00-0.260] ng/mL, < 0.001). KCNJ2 protein levels in BAL fluid were significantly higher in IPF (6.587 [1.441-26.01] ng/mL) than in NCs (0.084 [0.00-0.260] ng/mL, < 0.001). KCNJ2 protein levels in BAL fluid were significantly higher in IPF (6.587 [1.441-26.01] ng/mL) than in NCs (0.084 [0.00-0.260] ng/mL, < 0.001). KCNJ2 protein levels in BAL fluid were significantly higher in IPF (6.587 [1.441-26.01] ng/mL) than in NCs (0.084 [0.00-0.260] ng/mL, < 0.001). KCNJ2 protein levels in BAL fluid were significantly higher in IPF (6.587 [1.441-26.01] ng/mL) than in NCs (0.084 [0.00-0.260] ng/mL.
KCNJ2 may participate in the development of IPF, and its protein level may be a candidate diagnostic and therapeutic molecule for IPF.
成纤维细胞功能障碍是特发性肺纤维化（IPF）的主要发病机制。钾电压门控通道亚家族 J 成员 2（KCNJ2）在肌成纤维细胞的增殖和心脏纤维化的发展中发挥关键作用。
本研究旨在评估 KCNJ2 在 IPF 中的作用。
使用实时 PCR 测量来自 IPF 患者和正常对照（NCs）的成纤维细胞中的 mRNA 表达。通过 ELISA 测量来自 NCs（n=30）、IPF（n=30）、IPF（n=30）、IPF（n=30）、IPF（n=30）、IPF（n=30）的支气管肺泡灌洗液（BAL）中的蛋白浓度。
使用实时 PCR 测量来自 IPF 患者和正常对照（NCs）的成纤维细胞中的 mRNA 表达。通过 ELISA 测量来自 NCs（n=30）、IPF（n=30）、IPF（n=30）、IPF（n=30）、IPF（n=30）、IPF（n=30）的支气管肺泡灌洗液（BAL）中的蛋白浓度。
KCNJ2 可能参与 IPF 的发生，其蛋白水平可能是 IPF 的候选诊断和治疗分子。
