Guyomard C, Bouffechoux J, Bourniche J, Chesné C
BIOPREDIC, Rennes, France.
Cell Biol Toxicol. 1994 Dec;10(5-6):375-9. doi: 10.1007/BF00755785.
A cell kit named PREDISAFE based on the use of confluent rabbit fibroblastic cells has been designed to predict eye irritancy of cosmetic raw materials and formulations. The kit can be stored for a few days and/or shipped at room temperature. Cytotoxicity was estimated after 1 min or 15 min contact with test compounds using the neutral red release assay. For the 84 products tested, IC50 values gave intervals similar to classes defined from the Draize test, i.e., mild, moderate, severe and extreme irritancy.
一种名为PREDISAFE的细胞试剂盒，基于汇合的兔成纤维细胞的使用而设计，用于预测化妆品原料和配方的眼刺激性。该试剂盒可在室温下储存数天和/或运输。使用中性红释放试验，在与测试化合物接触1分钟或15分钟后评估细胞毒性。对于所测试的84种产品，半数抑制浓度（IC50）值给出的区间与从兔眼刺激试验定义的类别相似，即轻度、中度、重度和极重度刺激。
