Yumoto N, Araki A, Sumida T, Saito T, Taniguchi M, Mikata A
1st Department of Pathology, School of Medicine, Chiba University, Japan.
Virchows Arch. 1995;426(1):11-8. doi: 10.1007/BF00194693.
Ten cases of primary gastric malignant lymphoma (PGL) were investigated by immunohistochemical and molecular genetic analysis. These cases were diagnosed histopathologically as follicular small cleaved cell type (1 case), diffuse small cleaved cell type (3 cases) and diffuse large cell type (6 cases) based on the WF (Working Formulation) classification. Seven cases classified as small cleaved or diffuse large cell type belong to low (4 cases) or high (3 cases) grade MALT lymphoma according to Isaacson's classification. All PGL belonged to B lineage cells according to immunohistochemical study and immunoglobulin rearrangements. Rearrangements of TCR beta chain genes were observed in four of the ten cases. The possibility that the TCR beta rearrangements were caused by tumour-infiltrating T-cells (TILs) was supported by the following observations: the tumours did not show T- and B-cell biphenotype, TCR beta exhibited functional VDJ rearrangement and V beta usage pattern was not a neoplastic type. Analysis of the repertoire of the TCR beta chain in TILs revealed a common usage of V beta 2 in the above four cases, and furthermore, predominant usage of a particular beta chain composed of V beta 2-D beta 2.1-J beta 2.3 was observed in one of the four cases. These results indicate that the TILs of PGL have a restricted TCR repertoire.
对10例原发性胃恶性淋巴瘤（PGL）进行了免疫组织化学和分子遗传学分析。根据工作分类法（WF），这些病例在组织病理学上被诊断为滤泡性小裂细胞型（1例）、弥漫性小裂细胞型（3例）和弥漫性大细胞型（6例）。根据艾萨克森分类法，7例分类为小裂细胞型或弥漫性大细胞型的病例属于低级别（4例）或高级别（3例）黏膜相关淋巴组织淋巴瘤。根据免疫组织化学研究和免疫球蛋白重排，所有PGL均属于B淋巴细胞系。10例中有4例观察到TCRβ链基因重排。以下观察结果支持TCRβ重排由肿瘤浸润性T细胞（TILs）引起的可能性：肿瘤未显示T细胞和B细胞双表型，TCRβ表现出功能性VDJ重排，且Vβ使用模式不是肿瘤性类型。对TILs中TCRβ链库的分析显示，上述4例中Vβ2有共同使用情况，此外，4例中的1例观察到由Vβ2-Dβ2.1-Jβ2.3组成的特定β链的主要使用情况。这些结果表明，PGL的TILs具有受限的TCR库。
