Coughlin R T, Fish D, Mather T N, Ma J, Pavia C, Bulger P
Cambridge Biotech Corp., Worcester, Massachusetts 01605.
J Infect Dis. 1995 Apr;171(4):1049-52. doi: 10.1093/infdis/171.4.1049.
A vaccine consisting of purified Escherichia coli-expressed recombinant full-length outer surface proteins A (OspA) and B (OspB) and the saponin adjuvant QS21 was evaluated for protection against Borrelia burgdorferi infection. Eleven beagles were vaccinated twice and then challenged with 10 field-collected adult female Ixodes scapularis. Xenodiagnosis revealed that all 11 nonvaccinated control dogs and 2 of 10 vaccinated dogs were infected with B. burgdorferi. Six of 11 control dogs also developed fever (0.75 +/- 0.38 degrees C) and were lethargic. One of the control dogs also developed a limp. Both of the infected vaccinated dogs were asymptomatic. Thus, the vaccine prevented tick-vectored infection and associated symptoms of Lyme disease.
对一种由纯化的大肠杆菌表达的重组全长外膜蛋白A（OspA）和B（OspB）以及皂苷佐剂QS21组成的疫苗进行了评估，以确定其对伯氏疏螺旋体感染的保护作用。11只比格犬接种疫苗两次，然后用10只野外采集的成年雌性肩突硬蜱进行攻击。异种诊断显示，所有11只未接种疫苗的对照犬和10只接种疫苗的犬中的2只感染了伯氏疏螺旋体。11只对照犬中有6只也出现发热（0.75±0.38摄氏度）并伴有嗜睡。其中一只对照犬还出现跛行。两只感染的接种疫苗犬均无症状。因此，该疫苗预防了蜱传播的感染和莱姆病的相关症状。
