Ma J, Bulger P A, Dante S, Davis D R, Perilli-Palmer B, Coughlin R T
Cambridge Biotech Corp., Worcester, MA 01605.
J Infect Dis. 1995 Apr;171(4):909-15. doi: 10.1093/infdis/171.4.909.
Canine antibody responses to Lyme disease subunit vaccines and to natural borrelial infection were investigated. Vaccines were formulated with QS21 and outer surface proteins A (OspA) and B (OspB) derived from Borrelia burgdorferi B31. Vaccines containing QS21 and the lipoproteins gave 4-fold higher IgG1 and 8-fold higher IgG2 antibody responses than without QS21. Antisera to lipidated OspA or OspB vaccines containing QS21 had high antiborrelial activity against isolates B31 and CA-2-87, similar to those with a vaccine containing both OspA and OspB. Only the combination vaccine induced antiborrelial activity against heterologous isolates 24008 Fr and Borrelia garinii G25. Nonlipidated OspA- and OspB-based vaccines with QS21 elicited lower antibody and antiborrelial activity than did lipidated OspA and OspB vaccines; 49% of naturally exposed dogs had low titers to OspA or OspB. Thus, vaccines using lipidated OspA, OspB, and QS21 could induce higher antiborrelial activity than did natural exposure.
研究了犬对莱姆病亚单位疫苗和自然伯氏疏螺旋体感染的抗体反应。疫苗由QS21以及源自伯氏疏螺旋体B31的外膜蛋白A（OspA）和B（OspB）配制而成。含有QS21和脂蛋白的疫苗产生的IgG1抗体反应比不含QS21的疫苗高4倍，IgG2抗体反应高8倍。含有QS21的脂化OspA或OspB疫苗的抗血清对分离株B31和CA - 2 - 87具有高抗伯氏疏螺旋体活性，类似于含有OspA和OspB的疫苗。只有联合疫苗诱导了针对异源分离株24008 Fr和伽氏疏螺旋体G25的抗伯氏疏螺旋体活性。含QS21的非脂化OspA和OspB疫苗引发的抗体和抗伯氏疏螺旋体活性低于脂化OspA和OspB疫苗；49%自然暴露的犬对OspA或OspB的滴度较低。因此，使用脂化OspA、OspB和QS21的疫苗比自然暴露能诱导更高的抗伯氏疏螺旋体活性。
