Poulet S, Cole S T
Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
Arch Microbiol. 1995 Feb;163(2):87-95. doi: 10.1007/BF00381781.
The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was the triplet CGC, or CRR (where R is a purine), it seems likely that PGRS arose by means of triplet expansion, accounting for its polymorphism. Several copies of PGRS were linked to a conserved open reading frame. PGRS was used as the target sequence for the polymerase chain reaction in an attempt to develop a new typing technique.
通过定位、克隆和测序对在结核分枝杆菌染色体上发现的富含鸟嘌呤的多态性重复序列（PGRS）进行了表征。PGRS至少存在于26个位点，由共有序列CGGCGGCAA的许多串联重复组成。由于共有基序的核心是三联体CGC，即CRR（其中R是嘌呤），因此PGRS似乎是通过三联体扩增产生的，这解释了其多态性。PGRS的几个拷贝与一个保守的开放阅读框相连。PGRS被用作聚合酶链反应的靶序列，试图开发一种新的分型技术。
