通过Southern印迹杂交对越南患者的结核分枝杆菌菌株进行鉴定
Characterization of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization.
作者信息
Yuen L K, Ross B C, Jackson K M, Dwyer B
机构信息
Clinical Pathology Laboratory, Fairfield Infectious Diseases Hospital, Victoria, Australia.
出版信息
J Clin Microbiol. 1993 Jun;31(6):1615-8. doi: 10.1128/jcm.31.6.1615-1618.1993.
A total of 41 Mycobacterium tuberculosis strains from patients of Vietnamese origin were analyzed by Southern blot hybridization with two different probes, IS6110 (Otal, I., et al., J. Clin. Microbiol. 29:1252-1254, 1991; Ross, B. C., et al., J. Clin. Microbiol. 30:942-946, 1992; Thierry, D., et al., J. Clin. Microbiol. 28:2668-2673, 1990; van Soolingen, D., et al., J. Clin. Microbiol. 29:2578-2586, 1991) and pTBN12 (Ross, B. C., et al., J. Clin. Microbiol. 30:942-946, 1992). The restriction fragment patterns of nine of these strains were virtually identical when the pTBN12 probe was used; five strains had a single copy of IS6110, and four strains failed to hybridize with the IS6110 probe. This relatively high frequency of strains with no or one copy of IS6110 suggests that the usefulness of IS6110 for epidemiological study may be limited in certain populations.
采用两种不同的探针，即IS6110（奥塔尔，I.等人，《临床微生物学杂志》29:1252 - 1254，1991；罗斯，B.C.等人，《临床微生物学杂志》30:942 - 946，1992；蒂埃里，D.等人，《临床微生物学杂志》28:2668 - 2673，1990；范索林根，D.等人，《临床微生物学杂志》29:2578 - 2586，1991）和pTBN12（罗斯，B.C.等人，《临床微生物学杂志》30:942 - 946，1992），对总共41株来自越南患者的结核分枝杆菌菌株进行了Southern杂交分析。当使用pTBN12探针时，其中9株菌株的限制性片段模式几乎相同；5株菌株有单个拷贝的IS6110，4株菌株未能与IS6110探针杂交。IS6110无拷贝或只有一个拷贝的菌株出现频率相对较高，这表明在某些人群中，IS6110用于流行病学研究的实用性可能有限。
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