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通过原子力显微镜观察黄原胶的构象

Visualisation of xanthan conformation by atomic force microscopy.

作者信息

Moffat Jonathan, Morris Victor J, Al-Assaf Saphwan, Gunning A Patrick

机构信息

Asylum Research an Oxford Instruments Company, Halifax Rd., High Wycombe, Buckinghamshire, HP12 3SE, UK.

Institute of Food Research, Norwich Research Park, Norwich, NR4 7UA, UK.

出版信息

Carbohydr Polym. 2016 Sep 5;148:380-9. doi: 10.1016/j.carbpol.2016.04.078. Epub 2016 Apr 20.

DOI:10.1016/j.carbpol.2016.04.078
PMID:27185152
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4879867/
Abstract

Direct visual evidence obtained by atomic force microscopy demonstrates that when xanthan is adsorbed from aqueous solution onto the heterogeneously charged substrate mica, its helical conformation is distorted. Following adsorption it requires annealing for several hours to restore its ordered helical state. Once the helix state reforms, the AFM images obtained showed clear resolution of the periodicity with a value of 4.7nm consistent with the previously predicted models. In addition, the images also reveal evidence that the helix is formed by a double strand, a clarification of an ambiguity of the xanthan ultrastructure that has been outstanding for many years.

摘要

通过原子力显微镜获得的直接视觉证据表明，当黄原胶从水溶液吸附到异质带电底物云母上时，其螺旋构象会发生扭曲。吸附后，需要退火数小时才能恢复其有序的螺旋状态。一旦螺旋状态重新形成，获得的原子力显微镜图像就清晰地显示出周期分辨率，其值为4.7纳米，与先前预测的模型一致。此外，图像还揭示了螺旋由双链形成的证据，这澄清了多年来一直存在的黄原胶超微结构的一个模糊之处。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/fa793d8b73e9/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/6417c5b4b438/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/12c6daae418e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/3859faa5a73b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/3df3411ddfe2/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/e5f7dab4d979/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/e57bc034b324/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/fa793d8b73e9/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/6417c5b4b438/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/12c6daae418e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/3859faa5a73b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/3df3411ddfe2/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/e5f7dab4d979/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/e57bc034b324/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d49/4879867/fa793d8b73e9/gr7.jpg

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