Lohmann W, Wiegand W, Stolwijk T R, van Delft J L, van Best J A
Department of Biophysics, Justus-Liebig-University, Giessen, Germany.
Ophthalmologica. 1995;209(1):7-10. doi: 10.1159/000310565.
The endogenous fluorescence of human choroid, sclera, and retinal pigment epithelium (RPE) in normal tissue and tissue with uveal melanoma was studied in vitro by a non-invasive and non-destructive fluorescence technique which had previously been applied for the diagnostic evaluation of pigmented lesions of the skin. The fluorescence of the normal choroid is rather dark, the normal sclera exhibits blue fluorescence and the RPE bright yellow fluorescence due to deposits of lipofuscin. In choroidal melanoma, the lipofuscin granulae at the level of the retinal pigment epithelium are cleaved off above the tumour and broken up into small remnants. The fluorescence intensity emitted from the tumour is rather low which agrees with previous findings on skin melanomas. The results may become interesting for diagnostic evaluation of uveal melanomas, uveal naevi, and pigmented conjunctival tumours by endogenous fluorescence.
采用一种此前已用于皮肤色素沉着病变诊断评估的非侵入性、非破坏性荧光技术，在体外研究了正常组织以及患有葡萄膜黑色素瘤的组织中人类脉络膜、巩膜和视网膜色素上皮（RPE）的内源性荧光。正常脉络膜的荧光相当暗，正常巩膜呈现蓝色荧光，而由于脂褐素沉积，视网膜色素上皮呈现亮黄色荧光。在脉络膜黑色素瘤中，视网膜色素上皮水平的脂褐素颗粒在肿瘤上方被切断，并分解成小的残余物。肿瘤发出的荧光强度相当低，这与之前关于皮肤黑色素瘤的研究结果一致。这些结果对于通过内源性荧光对葡萄膜黑色素瘤、葡萄膜痣和色素性结膜肿瘤进行诊断评估可能具有重要意义。
