Aznar J, Safi H, Romero J, Alejo A, Gracia A, Palomares J C
Department of Microbiology, University of Seville, Spain.
Pediatr Infect Dis J. 1995 Jan;14(1):44-8. doi: 10.1097/00006454-199501000-00009.
Analysis of restriction fragment length polymorphisms is a well-established method of "DNA fingerprinting" that has been used to trace the transmission of particular strains of Mycobacterium tuberculosis during investigations of outbreaks. This report describe the use of restriction fragment length polymorphisms and arbitrarily primed polymerase chain reaction analysis to investigate two outbreaks of tuberculosis that affected six children who attended two pediatric wards in our hospital. In both outbreaks a history of household exposure to an adult with M. tuberculosis was obtained and suspected tuberculous contacts were identified. We have demonstrated unequivocally the strain relationship among the isolates in all the cases by restriction fragment length polymorphisms and arbitrarily primed polymerase chain reaction analysis. These techniques are very useful for performing epidemiologic studies of tuberculosis in children where natural history of tuberculosis infection is different from that in adults in that it is almost always primary infection rather than reactivation.
限制性片段长度多态性分析是一种成熟的“DNA指纹识别”方法，已被用于在结核病暴发调查期间追踪特定结核分枝杆菌菌株的传播。本报告描述了使用限制性片段长度多态性和任意引物聚合酶链反应分析来调查两起结核病暴发事件，这两起事件影响了在我院两个儿科病房就诊的6名儿童。在这两起暴发事件中，均获得了家庭中接触成年结核分枝杆菌患者的病史，并确定了疑似结核接触者。通过限制性片段长度多态性和任意引物聚合酶链反应分析，我们明确证实了所有病例中分离株之间的菌株关系。这些技术对于开展儿童结核病的流行病学研究非常有用，因为儿童结核病感染的自然史与成人不同，儿童几乎总是原发性感染而非再激活感染。
