Truett G E, Jacob H J, Miller J, Drouin G, Bahary N, Smoller J W, Lander E S, Leibel R L
Laboratory of Human Behavior and Metabolism, Rockefeller University, New York, New York 10021.
Mamm Genome. 1995 Jan;6(1):25-30. doi: 10.1007/BF00350889.
Thirteen loci, including the obesity gene fatty (fa), were incorporated into a linkage map of rat Chromosome (Chr) 5. These loci were mapped in obese (fa/fa) progeny of a cross between BN x 13M-fal+F1 animals. Obese rats were scored for BN and 13M alleles at four loci (Ifna, D1S85h, C8b, and Lck1) by restriction fragment length polymorphisms and at eight additional loci (Glut1, Sv4j2, R251, R735, R980, R252, R371, and R1138) by simple sequence length polymorphisms (SSLP). The resulting map spans 67.3 cM of Chr5, presenting nine previously unmapped loci and one locus (Lck1) previously assigned to Chr 5 by use of somatic cell hybrid lines. Seven of the eight SSLP loci are newly identified; the SSLP linkage group alone spans 56.8 cM. The order of the loci is Sv4j2-R251-R735-R980-R1138-Ifna-fa-+ ++D1S85h-C8b-(Glut1-R252-R371)-Lck1. One locus, D1S85h, was found to lie only 0.4 cM from fa, close enough to serve as a reliable marker for the prediction of phenotype from genotype, and will be useful also for studies on the development of obesity in the fatty rat.
包括肥胖基因fatty（fa）在内的13个基因座被整合到大鼠5号染色体（Chr）的连锁图谱中。这些基因座在BN×13M-fal+F1动物杂交后代的肥胖（fa/fa）子代中进行定位。通过限制性片段长度多态性对肥胖大鼠的四个基因座（Ifna、D1S85h、C8b和Lck1）的BN和13M等位基因进行评分，并通过简单序列长度多态性（SSLP）对另外八个基因座（Glut1、Sv4j2、R251、R735、R980、R252、R371和R1138）进行评分。所得图谱跨越5号染色体的67.3 cM，呈现出九个先前未定位的基因座和一个先前通过体细胞杂交系定位到5号染色体的基因座（Lck1）。八个SSLP基因座中有七个是新鉴定的；仅SSLP连锁群就跨越56.8 cM。基因座的顺序为Sv4j2-R251-R735-R980-R1138-Ifna-fa-+++D1S85h-C8b-（Glut1-R252-R371）-Lck1。发现一个基因座D1S85h与fa的距离仅为0.4 cM，近到足以作为从基因型预测表型的可靠标记，并且对于研究肥胖大鼠肥胖症的发展也将是有用的。
