Goldmuntz E A, Remmers E F, Zha H, Mathern P, Du Y, Crofford L J, Wilder R L
Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Mamm Genome. 1993 Nov;4(11):670-5. doi: 10.1007/BF00360905.
Seven polymorphic markers comprising a single linkage group were assigned to rat Chromosome (Chr) 5 by linkage analysis of the progeny of an F2 intercross of Fischer (F344/N) and Lewis (LEW/N) inbred rats. Three genes, alpha-L-fucosidase 1 (FUCA1), mitochondrial superoxide dismutase (SOD2), and glucose transporter (GLUT1), were mapped by restriction fragment length polymorphism (RFLP) analysis. Two genes, glucose transporter (GTG3) and elastase II (ELAII), one pseudogene for alpha tubulin (TUBAPS), and one sequence related to the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene (PFKFBP1-related sequence) were mapped by simple sequence repeat (SSR) polymorphism analysis. The loci are in the following order: SOD2, GTG3/GLUT1, FUCA1, ELAII/PFKFBP1-related sequence, and TUBAPS. This linkage group covered 68.3 cM of rat Chr 5. The SSR markers were highly polymorphic in 13 inbred rat strains (SHR/N, WKY/N, MNR/N, MR/N, LOU/MN, BN/SsN, BUF/N, WBB1/N, WBB2/N, ACI/N, LER/N, F344/N, and LEW/N). These markers, located on rat Chr 5, will be useful in genetic studies of inbred rats.
通过对Fischer（F344/N）和Lewis（LEW/N）近交系大鼠F2代杂交后代进行连锁分析，将包含一个单一连锁群的7个多态性标记定位到大鼠第5号染色体（Chr 5）上。通过限制性片段长度多态性（RFLP）分析对三个基因，即α-L-岩藻糖苷酶1（FUCA1）、线粒体超氧化物歧化酶（SOD2）和葡萄糖转运蛋白（GLUT1）进行了定位。通过简单序列重复（SSR）多态性分析对两个基因，即葡萄糖转运蛋白（GTG3）和弹性蛋白酶II（ELAII）、一个α微管蛋白假基因（TUBAPS）以及一个与6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶基因相关的序列（PFKFBP1相关序列）进行了定位。这些位点的排列顺序如下：SOD2、GTG3/GLUT1、FUCA1、ELAII/PFKFBP1相关序列和TUBAPS。这个连锁群覆盖了大鼠Chr 5的68.3厘摩。SSR标记在13个近交系大鼠品系（SHR/N、WKY/N、MNR/N、MR/N、LOU/MN、BN/SsN、BUF/N、WBB1/N、WBB2/N、ACI/N、LER/N、F344/N和LEW/N）中具有高度多态性。这些位于大鼠Chr 5上的标记将有助于近交系大鼠的遗传学研究。
