编码Rab19（一种新型组织特异性小GTP酶）的小鼠cDNA克隆的分离。

Isolation of a murine cDNA clone encoding Rab19, a novel tissue-specific small GTPase.

作者信息

Lütcke A, Olkkonen V M, Dupree P, Lütcke H, Simons K, Zerial M

机构信息

Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.

出版信息

Gene. 1995 Apr 3;155(2):257-60. doi: 10.1016/0378-1119(94)00931-h.

DOI:10.1016/0378-1119(94)00931-h

PMID:7721101

Abstract

Using a rapid amplification of cDNA ends (RACE) cloning approach, we have isolated a cDNA clone encoding Rab19, a novel small GTPase of the Rab subfamily contained within partial sequences previously described [Chavrier et al., Gene 112 (1992) 261-264]. Northern blot analysis of the distribution of the rab19 mRNA in various adult mouse tissues and NIH 3T3 fibroblasts revealed that rab19 is expressed in a tissue-specific manner. The rab19 transcript was detected at high levels in intestine, lung and spleen, and at a lower level in kidney. In contrast, liver, brain, heart and NIH 3T3 fibroblasts contain only very little or no detectable rab19 mRNA. Therefore, Rab19 is likely to represent a novel tissue- or cell type-specific small GTPase.

摘要

利用cDNA末端快速扩增（RACE）克隆方法，我们分离出一个编码Rab19的cDNA克隆，Rab19是Rab亚家族的一种新型小GTP酶，此前在部分序列中已有描述[Chavrier等人，《基因》112（1992）261 - 264]。对成年小鼠各种组织和NIH 3T3成纤维细胞中rab19 mRNA分布的Northern印迹分析表明，rab19以组织特异性方式表达。在小肠、肺和脾脏中检测到高水平的rab19转录本，在肾脏中的水平较低。相比之下，肝脏、大脑、心脏和NIH 3T3成纤维细胞中仅含有极少或无法检测到的rab19 mRNA。因此，Rab19可能代表一种新型的组织或细胞类型特异性小GTP酶。