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用于扫描电子显微镜检查的血管内皮制备:灌注固定和浸入固定效果的比较

Preparation of vascular endothelium for scanning electron microscopy: a comparison of the effects of perfusion and immersion fixation.

作者信息

Gertz S D, Rennels M L, Forbes M S

出版信息

J Microsc. 1975 Dec;105(3):309-13. doi: 10.1111/j.1365-2818.1975.tb04065.x.

DOI:10.1111/j.1365-2818.1975.tb04065.x
PMID:772214
Abstract

A comparison of currently used methods of tissue fixation for scanning electron microscopic study of vascular endothelium revealed that in situ fixation by intravascular perfusion is superior to immersion for the preservation of endothelial surface morphology.

摘要

对目前用于血管内皮扫描电子显微镜研究的组织固定方法进行比较后发现,通过血管内灌注进行原位固定在保存内皮表面形态方面优于浸入固定。

相似文献

1
Preparation of vascular endothelium for scanning electron microscopy: a comparison of the effects of perfusion and immersion fixation.用于扫描电子显微镜检查的血管内皮制备:灌注固定和浸入固定效果的比较
J Microsc. 1975 Dec;105(3):309-13. doi: 10.1111/j.1365-2818.1975.tb04065.x.
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Scanning electron microscopy - some methodological considerations.扫描电子显微镜术——一些方法学上的考量
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A scanning electron microscope study on the endothelium of the vessels. I. Fine structure of the endothelial surface of aorta and some other arteries in normal rabbits.血管内皮的扫描电子显微镜研究。I. 正常兔主动脉及其他一些动脉内皮表面的精细结构。
Arch Histol Jpn. 1974 Jul;37(1):1-14.
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Luminal surface of distended arteries by scanning electron microscopy: eliminating configurational and technical artefacts.通过扫描电子显微镜观察扩张动脉的腔面:消除形态和技术假象
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The influence of 5-fluorouracil on the endothelium in small arteries. An electron microscopic study in rabbits.5-氟尿嘧啶对小动脉内皮的影响。家兔的电子显微镜研究。
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Electron microscopy of endothelial microvilli of large arteries.大动脉内皮微绒毛的电子显微镜检查。
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Repair in arterial tissue. A semiquantitative scanning-electron-microscopic and light-microscopic study on the effects of toxic doses of noradrenaline on the endothelium of the rabbit thoracic aorta.动脉组织的修复。关于去甲肾上腺素中毒剂量对兔胸主动脉内皮影响的半定量扫描电子显微镜和光学显微镜研究。
Blood Vessels. 1977 Mar;14(2):116-27.
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Model system for the study of inital damage to arterial endothelial cells in situ by scanning electron microscopy.通过扫描电子显微镜对动脉内皮细胞原位初始损伤进行研究的模型系统。
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Glomerular endothelial injury related to renal perfusion. A scanning electron microscopic study.与肾灌注相关的肾小球内皮损伤。一项扫描电子显微镜研究。
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PLoS One. 2016 May 19;11(5):e0155824. doi: 10.1371/journal.pone.0155824. eCollection 2016.
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Morphology of arteries, veins, and capillaries in cancer of the larynx: scanning electron-microscopical study on microcorrosion casts.喉癌中动脉、静脉和毛细血管的形态学:微腐蚀铸型的扫描电子显微镜研究
J Cancer Res Clin Oncol. 1981;100(3):271-83. doi: 10.1007/BF00410688.
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The thermic vascular anastomosis (TVA). IV. Analysis by scanning electron microscopy.
Acta Neurochir (Wien). 1983;67(1-2):139-53. doi: 10.1007/BF01401674.
4
Haemodynamically-induced intimal tears in experimental U-shaped arterial loops as seen by scanning electron microscopy.扫描电子显微镜观察到的实验性U形动脉袢中血流动力学诱导的内膜撕裂。
Br J Exp Pathol. 1985 Oct;66(5):577-84.
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Aneurysm of the rabbit common carotid artery induced by periarterial application of calcium chloride in vivo.氯化钙动脉周围体内注射诱导兔颈总动脉瘤形成
J Clin Invest. 1988 Mar;81(3):649-56. doi: 10.1172/JCI113368.
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Cellular pathology of the rat aorta. Pseudo-vacuoles and myo-endothelial herniae.大鼠主动脉的细胞病理学。假空泡与肌内皮疝
Virchows Arch A Pathol Anat Histol. 1979 Jul 31;383(2):135-48. doi: 10.1007/BF01200895.