Greenhill N S, Stehbens W E
Br J Exp Pathol. 1985 Oct;66(5):577-84.
Experimentally-induced U-shaped carotid loops, simulating arterial tortuosities and kinks were examined by scanning electron microscopy to seek flow-induced changes in the intimal surface. In 14 New Zealand white rabbits carotid arterial transplants were fashioned into U-shaped loops by microvascular surgery. The rabbits were sacrificed from 4 to 226 days post-operatively. Tears in the internal elastic lamina occurred in all loops from 5 days post-operatively and were predominantly transverse and localised about the greater curvature of the bends of each loop. Though initially denuded, all tears appeared endothelialised after 6 days, coalescing later as they increased in size and extent. In older animals only islands of wrinkled internal elastic lamina remained at those sites. Endothelial cells in the tears were small, numerous and polyhedral with raised nuclei. The lesser curvature of the three bends in the loops displayed some irregular wrinkling of the internal elastic lamina. In the three oldest animals a few longitudinal tears were observed on the lesser curvature of the main bend. The specific localization of intimal tears supports the concept that they were hemodynamically induced.
通过扫描电子显微镜检查实验诱导的U形颈动脉袢，模拟动脉迂曲和扭结，以寻找血流诱导的内膜表面变化。在14只新西兰白兔中，通过微血管手术将颈动脉移植制成U形袢。术后4至226天处死兔子。术后5天所有袢的内弹性膜均出现撕裂，主要为横向撕裂，且局限于每个袢弯曲的大曲率处。虽然最初内膜剥脱，但所有撕裂在6天后似乎都有内皮化，随着其大小和范围的增加，后来融合在一起。在老年动物中，只有起皱的内弹性膜岛留在这些部位。撕裂处的内皮细胞小、数量多且呈多面体，细胞核凸起。袢中三个弯曲的小曲率处显示内弹性膜有一些不规则的起皱。在三只年龄最大的动物中，在主弯曲的小曲率处观察到一些纵向撕裂。内膜撕裂的特定定位支持了它们是由血流动力学诱导的这一概念。
