Gertz S D, Kurgan A, Eisenberg D
Department of Anatomy, Hebrew University, Jerusalem, Israel.
J Clin Invest. 1988 Mar;81(3):649-56. doi: 10.1172/JCI113368.
Experimental aneurysmatic dilatation of the rabbit common carotid artery was induced by a single, periarterial application of calcium chloride in vivo. Vessels were fixed in situ after 3 d, 1 wk, 3 wk, 6 wk, and 12 wk by intracardiac perfusion of glutaraldehyde and tissues prepared for light, scanning, and transmission electron microscopy. Progressive focal aneurysmal dilatation was seen limited to the site of calcium application with endothelial damage and thrombus formation in areas of irregular luminal contour. Disruption of the elastic network of the intima and media was seen with varying degrees of intimal fibromuscular hyperplasia and medial disorganization. The calcium-elastic tissue complex was the focus of the inflammatory, arteriosclerotic reaction and subsequent aneurysm formation. The inflammatory cell infiltration initially included primarily neutrophils followed by lymphocytes, plasma cells, monocytes, and multinucleated giant cells. These studies support the hypothesis that disruption of the elastic tissue network of the vascular wall represents an important pathogenetic factor in the initiation of aneurysmal dilatation. In addition, the results of these studies suggest that interaction of calcium with the elastica of the arterial wall may represent an important pathogenetic factor in the initiation of giant cell arteritis.
通过在兔颈总动脉周围单次局部应用氯化钙在体内诱导实验性动脉瘤扩张。在3天、1周、3周、6周和12周后，通过心脏内灌注戊二醛原位固定血管，并制备用于光镜、扫描电镜和透射电镜检查的组织。可见渐进性局灶性动脉瘤扩张局限于氯化钙应用部位，在管腔轮廓不规则区域有内皮损伤和血栓形成。可见内膜和中膜弹性网络破坏，伴有不同程度的内膜纤维肌增生和中膜紊乱。钙-弹性组织复合物是炎症、动脉硬化反应及随后动脉瘤形成的焦点。炎症细胞浸润最初主要包括中性粒细胞，随后是淋巴细胞、浆细胞、单核细胞和多核巨细胞。这些研究支持这样的假说，即血管壁弹性组织网络的破坏是动脉瘤扩张起始的一个重要致病因素。此外,这些研究结果表明，钙与动脉壁弹性组织的相互作用可能是巨细胞动脉炎起始的一个重要致病因素。
