Kusukawa J, Sasaguri Y, Morimatsu M, Kameyama T
Department of Oral and Maxillofacial Surgery, Kurume University School of Medicine, Japan.
J Oral Maxillofac Surg. 1995 May;53(5):530-4. doi: 10.1016/0278-2391(95)90065-9.
The object of this study was to evaluate the significance of matrix metalloproteinase-3 (MMP-3) in tumor invasion and metastasis of early squamous cell carcinoma (SCC) of the oral cavity.
Surgical specimens from 65 patients with stage I and II SCC of the oral cavity were the subjects of this study. Tissue specimens were fixed in formalin and embedded in paraffin, and the sections were stained with monospecific antibodies against human MMP-3 by the avidin-biotin-peroxidase complex method.
Of the 65 patients, 30 (46.2%) tested positive for MMP-3. Immunoreactivity revealed the expression of MMP-3 to be in the small cancer nests in the advancing front of invasion, but not in normal oral epithelium. MMP-3 expression was positively correlated with tumor size, depth of tumor invasion, diffuse invasive mode, and the high incidence of lymph node metastasis.
MMP-3-containing tumors will invade adjacent normal tissues more aggressively, including lymphatic and blood vessels. Therefore, the examination of MMP-3 expression in biopsy specimens should provide information useful in predicting the malignant potential of early SCC of the oral cavity.
本研究的目的是评估基质金属蛋白酶-3（MMP-3）在口腔早期鳞状细胞癌（SCC）肿瘤侵袭和转移中的意义。
本研究的对象为65例口腔I期和II期SCC患者的手术标本。组织标本用福尔马林固定，石蜡包埋，切片采用抗人MMP-3单特异性抗体，通过抗生物素蛋白-生物素-过氧化物酶复合物法染色。
65例患者中，30例（46.2%）MMP-3检测呈阳性。免疫反应显示MMP-3的表达位于侵袭前沿的小癌巢中，而在正常口腔上皮中未表达。MMP-3表达与肿瘤大小、肿瘤侵袭深度、弥漫性侵袭方式以及淋巴结转移的高发生率呈正相关。
含MMP-3的肿瘤将更具侵袭性地侵犯相邻正常组织，包括淋巴管和血管。因此，检测活检标本中MMP-3的表达应有助于预测口腔早期SCC的恶性潜能。
