Jenkinson H F, Lala H C, Shepherd M G
Department of Oral Biology, University of Otago, Dunedin, New Zealand.
Infect Immun. 1990 May;58(5):1429-36. doi: 10.1128/iai.58.5.1429-1436.1990.
Thirteen strains of viridans group streptococci and two strains of other streptococci were tested for coaggregation with Candida albicans. Streptococcus sanguis strains generally exhibited low levels of adherence to 28 degrees C-grown exponential-phase yeast cells, but starvation of yeast cells for glucose at 37 degrees C (or at 28 degrees C) increased their coaggregating activity with these streptococci by at least tenfold. This was a property common to four C. albicans strains tested, two of which were able to form mycelia (6406 and MEN) and two of which were not (MM2002 and CA2). The expression of the coaggregation adhesin during yeast cell starvation was inhibited by addition of trichodermin or amphotericin B. The strains of S. sanguis, Streptococcus gordonii, and Streptococcus oralis tested for coaggregating activity encompassed a diverse range of physiological and morphological types, yet all exhibited saturable coaggregation with starved C. albicans cells. There was no correlation of cell surface hydrophobicity, of either yeast or streptococcal cells, with their abilities to coaggregate. Strains of Streptococcus anginosus also coaggregated with starved yeast cells; Streptococcus salivarius and Streptococcus pyogenes coaggregated to a lesser degree with C. albicans, and the coaggregation with S. pyogenes was not promoted by yeast cell starvation; Streptococcus mutans and Enterococcus faecalis did not coaggregate with yeast. The coaggregation reactions of S. sanguis and S. gordonii with C. albicans were inhibited by EDTA and by heat or protease treatment of the yeast cells and were not reversible by the addition of lactose or other simple sugars. These observations extend the range of intergeneric coaggregations that are known to occur between oral microbes and suggest that coaggregations of C. albicans with viridans group streptococci may be important for colonization of oral surfaces by the yeast.
对13株草绿色链球菌和2株其他链球菌进行了与白色念珠菌共聚集的测试。血链球菌菌株通常对在28℃培养的指数生长期酵母细胞的黏附水平较低，但在37℃（或28℃）使酵母细胞缺乏葡萄糖会使其与这些链球菌的共聚集活性至少提高10倍。这是所测试的4株白色念珠菌菌株共有的特性，其中2株能够形成菌丝体（6406和MEN），另外2株则不能（MM2002和CA2）。在酵母细胞饥饿期间共聚集黏附素的表达可被曲古抑菌素或两性霉素B抑制。测试共聚集活性的血链球菌、戈登链球菌和口腔链球菌菌株涵盖了多种生理和形态类型，但所有菌株与饥饿的白色念珠菌细胞均表现出饱和共聚集。酵母或链球菌细胞的表面疏水性与其共聚集能力之间没有相关性。咽峡炎链球菌菌株也与饥饿的酵母细胞共聚集；唾液链球菌和化脓性链球菌与白色念珠菌的共聚集程度较低，且酵母细胞饥饿不会促进化脓性链球菌的共聚集；变形链球菌和粪肠球菌不与酵母共聚集。血链球菌和戈登链球菌与白色念珠菌的共聚集反应可被EDTA以及对酵母细胞进行加热或蛋白酶处理所抑制，且添加乳糖或其他单糖不能使其逆转。这些观察结果扩展了已知的口腔微生物之间发生的属间共聚集范围，并表明白色念珠菌与草绿色链球菌的共聚集可能对该酵母在口腔表面的定殖很重要。
