Deng W, Gordon M P, Nester E W
Department of Microbiology, University of Washington, Seattle 98195, USA.
J Bacteriol. 1995 May;177(9):2554-9. doi: 10.1128/jb.177.9.2554-2559.1995.
Two novel insertion sequences, IS1312 and IS1313, were found in pTiBo542, the Ti plasmid of Agrobacterium tumefaciens strains Bo542 and A281. Nucleotide sequencing and Southern hybridization revealed that IS1312 and IS1313 are homologous to Rhizobium meliloti ISRm1 and ISRm2, respectively. IS1312, ISRm1, and another Agrobacterium insertion sequence, IS426, belong to the same IS3 family of insertion sequences; however, IS1312 is more closely related to the Rhizobium ISRm1 than it is to the Agrobacterium IS426. The distribution patterns of these insertion elements and their sequence similarities suggest that IS1312 and IS1313 were horizontally transferred from R. meliloti to A. tumefaciens.
在根癌土壤杆菌菌株Bo542和A281的Ti质粒pTiBo542中发现了两个新的插入序列IS1312和IS1313。核苷酸测序和Southern杂交显示，IS1312和IS1313分别与苜蓿根瘤菌的ISRm1和ISRm2同源。IS1312、ISRm1以及另一个土壤杆菌插入序列IS426属于插入序列的同一个IS3家族；然而，IS1312与根瘤菌的ISRm1的关系比与土壤杆菌的IS426更为密切。这些插入元件的分布模式及其序列相似性表明，IS1312和IS1313是从苜蓿根瘤菌水平转移到根癌土壤杆菌的。
