Yokota K, Kanamoto R, Kito M
J Bacteriol. 1980 Mar;141(3):1047-51. doi: 10.1128/jb.141.3.1047-1051.1980.
The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with phospholipase C. No significant difference in the composition of the molecular species was found between the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties. On the other hand, the composition of the molecular species of phosphatidylglycerol was different from that of cardiolipin. Phosphatidylglycerol contained more of the 1-palmitoyl 2-cis-9,10-methylenehexadecanoyl and 1-palmitoyl 2-cis-11,12-methyleneoctadecanoyl species than did cardiolipin. The difference in the composition of the molecular species between cardiolipin and phosphatidylglycerol may depend on the difference in the turnover rates of both phospholipids.
测定了大肠杆菌B在37℃指数生长后期酸性磷脂分子种类的组成。通过磷脂酶C有限水解分离出心磷脂的两个磷脂酰基团，即心磷脂的3-(3-sn-磷脂酰)和1-(3-sn-磷脂酰)部分。在3-(3-sn-磷脂酰)和1-(3-sn-磷脂酰)部分之间，未发现分子种类组成有显著差异。另一方面，磷脂酰甘油的分子种类组成与心磷脂不同。磷脂酰甘油比心磷脂含有更多的1-棕榈酰-2-顺式-9,10-亚甲基十六烷酰基和1-棕榈酰-2-顺式-11,12-亚甲基十八烷酰基种类。心磷脂和磷脂酰甘油分子种类组成的差异可能取决于两种磷脂周转率的差异。
