van der Giessen M, Homan W L, van Kernbeek G, Aalberse R C, Dieges P H
Int Arch Allergy Appl Immunol. 1976;50(5):625-40. doi: 10.1159/000231566.
The subclasses of IgG antibodies formed by grass pollen-allergic patients during immunotherapy were investigated by using a radioallergosorbent test (RAST) and a quantitative immunofluorescence method known as the defined antigen substrate spheres (DASS) system. By the use of rabbit antisera directed against the subclasses of IgG, the specificity of which was checked in the passive hemagglutination and immunofluorescence techniques, it was shown that a relatively high proportion of the grass pollen-specific antibodies belonged to the IgG4 subclass. Apart from the high binding activity of IgG4 which increased during treatment, a moderate binding activity of the other subclasses was also found. Binding of all subclasses increased slightly in the pollen season and could be specifically blocked by perincubation with soluble grass pollen extract. The results of the IgG4 binding, determined in vitro with the DASS system, and the blocking activity of the sera, determined in vivo by skin tests are suggestive for a relation between these activities. Also in the group of patients with a low IgE-RAST score, the skin reactivity decreased as the IgG4 binding activity increased.
通过放射变应原吸附试验（RAST）和一种称为限定抗原底物球（DASS）系统的定量免疫荧光方法，对草花粉过敏患者在免疫治疗期间形成的IgG抗体亚类进行了研究。通过使用针对IgG亚类的兔抗血清，其特异性在被动血凝和免疫荧光技术中得到了验证，结果表明，相对较高比例的草花粉特异性抗体属于IgG4亚类。除了治疗期间IgG4的高结合活性增加外，还发现其他亚类具有中等结合活性。在花粉季节，所有亚类的结合略有增加，并且可以通过与可溶性草花粉提取物预温育而被特异性阻断。用DASS系统在体外测定的IgG4结合结果以及通过皮肤试验在体内测定的血清阻断活性提示了这些活性之间的关系。同样在低IgE-RAST评分的患者组中，随着IgG-4结合活性的增加，皮肤反应性降低。
