Merritt K, Treadwell T L, Jacobs N J
J Clin Microbiol. 1976 Mar;3(3):287-90. doi: 10.1128/jcm.3.3.287-290.1976.
Streptococci from clinical isolates were studied for their ability to produce pigment in stab cultures in Columbia agar. Serological grouping of these organisms was done by counterimmunoelectrophoresis using Burroughs-Wellcome antisera. In this group of isolates, 66 of the 68 organisms grouped as B by serological testing produced pigment in the Columbia agar stab cultures. Pigment was not produced by any of the other 36 streptococci studied (11 group A, 9 group C, 4 group D, and 12 nongroupable). The use of the Columbia agar stab culture is recommended as a rapid and simple test for recognition of group B streptococci. The counterimmunoelectrophoresis test is also suggested as a convenient, rapid, and sensitive method for grouping the streptococci.
对临床分离出的链球菌进行研究，观察其在哥伦比亚琼脂穿刺培养物中产生色素的能力。使用百时美施贵宝抗血清通过对流免疫电泳对这些微生物进行血清学分型。在这组分离株中，68株经血清学检测归为B组的微生物中有66株在哥伦比亚琼脂穿刺培养物中产生色素。其他36株研究的链球菌（11株A组、9株C组、4株D组和12株无法分型）均未产生色素。推荐使用哥伦比亚琼脂穿刺培养作为识别B组链球菌的快速简便检测方法。还建议将对流免疫电泳试验作为对链球菌进行分型的便捷、快速且灵敏的方法。
