De La Rosa M, Villareal R, Vega D, Miranda C, Martinezbrocal A
J Clin Microbiol. 1983 Oct;18(4):779-85. doi: 10.1128/jcm.18.4.779-785.1983.
A new starch serum medium, Granada medium, for isolation and identification of group B streptococci (GBS) anaerobically as red colonies is described. The medium contains 3.8% Proteose Peptone no. 3 (Difco), 15% soluble starch 1252 (Merck), 10% coagulated horse serum, 15 micrograms of trimethoprim per ml, and 0.06 M phosphate buffer (pH 7.8; medium pH 7.4). This medium inhibited fecal flora and at the same time supported growth of GBS. A new pigment-enhancing effect of folate inhibitors on GBS is reported and used in the formulation of the medium. The good selective and differential properties of the Granada medium favor quicker and easier detection of GBS in heavily contaminated specimens. Since the medium is convenient to use and requires only 18 h of incubation to detect and identify GBS, it should be useful in any clinical microbiology laboratory and would assist in the early detection of GBS in clinical specimens.
本文描述了一种新的淀粉血清培养基——格拉纳达培养基，用于厌氧分离和鉴定B族链球菌（GBS），其菌落呈红色。该培养基含有3.8%的3号蛋白胨（Difco）、15%的可溶性淀粉1252（默克）、10%的凝固马血清、每毫升15微克的甲氧苄啶以及0.06M磷酸盐缓冲液（pH 7.8；培养基pH 7.4）。这种培养基能抑制粪便菌群，同时支持GBS生长。本文报道了叶酸抑制剂对GBS的一种新的色素增强作用，并将其用于培养基的配方中。格拉纳达培养基良好的选择性和鉴别特性有利于在严重污染的标本中更快、更轻松地检测GBS。由于该培养基使用方便，检测和鉴定GBS仅需18小时培养，因此它应在任何临床微生物实验室中都有用，并有助于临床标本中GBS的早期检测。
