Colombo A L, Barchiesi F, McGough D A, Rinaldi M G
Department of Pathology, University of Texas Health Science Center, San Antonio, USA.
J Clin Microbiol. 1995 Mar;33(3):535-40. doi: 10.1128/jcm.33.3.535-540.1995.
The use of Etest strips for antimicrobial susceptibility testing is a new and promising method with broad applications in microbiology. Since these strips contain a predefined continuous gradient of a drug, it is possible to obtain a reproducible, quantitative MIC reading. We performed a prospective and double-blinded study to compare the Etest and National Committee for Clinical Laboratory Standards (Villanova, Pa.) broth macrodilution methods for determining the MICs of fluconazole, itraconazole, and ketoconazole for 100 clinical isolates (25 Candida albicans, 25 Cryptococcus neoformans var. neoformans, 20 Torulopsis [Candida] glabrata, 15 Candida tropicalis, and 15 Candida parapsilosis). The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-P guidelines. Despite differences between results for some species-drug combinations, Etest and macrobroth MICs were, in general, in good agreement. The MIC agreement rates for the two methods, within +/- 1 dilution, were 71% for ketoconazole, 80% for fluconazole, and 84% for itraconazole. According to our data, Etest has potential utility as an alternative method.
使用Etest试纸条进行抗菌药敏试验是一种新的且有前景的方法，在微生物学领域有广泛应用。由于这些试纸条含有预定义的药物连续梯度，因此有可能获得可重复的定量最低抑菌浓度（MIC）读数。我们进行了一项前瞻性双盲研究，比较Etest法与美国国家临床实验室标准委员会（宾夕法尼亚州维拉诺瓦）的肉汤稀释法，以测定100株临床分离株（25株白色念珠菌、25株新生隐球菌新生变种、20株光滑念珠菌、15株热带念珠菌和15株近平滑念珠菌）对氟康唑、伊曲康唑和酮康唑的MIC。Etest法按照制造商的说明进行，参考方法按照美国国家临床实验室标准委员会M27-P文件指南进行。尽管某些菌种-药物组合的结果存在差异，但总体而言，Etest法和肉汤稀释法测定的MIC结果具有良好的一致性。两种方法在±1个稀释度内的MIC一致率分别为：酮康唑71%、氟康唑80%、伊曲康唑84%。根据我们的数据，Etest法有作为替代方法的潜在用途。
