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J Clin Microbiol. 1996 Feb;34(2):482-3. doi: 10.1128/jcm.34.2.482-483.1996.
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Diagnosis of cytomegalovirus infection of the nervous system in AIDS by polymerase chain reaction analysis of cerebrospinal fluid.通过脑脊液聚合酶链反应分析诊断艾滋病患者的神经系统巨细胞病毒感染
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Level of cytomegalovirus (CMV) DNA in cerebrospinal fluid of subjects with AIDS and CMV infection of the central nervous system.患有艾滋病且中枢神经系统感染巨细胞病毒（CMV）的受试者脑脊液中巨细胞病毒（CMV）DNA的水平。
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引用本文的文献

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Quantitation of cytomegalovirus: methodologic aspects and clinical applications.巨细胞病毒的定量分析：方法学方面及临床应用
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Unusual course of herpes simplex virus encephalitis after acyclovir therapy.阿昔洛韦治疗后单纯疱疹病毒性脑炎的不寻常病程。
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本文引用的文献

1
Polymerase chain reaction to detect cytomegalovirus DNA in the cerebrospinal fluid of neonates with congenital infection.聚合酶链反应检测先天性感染新生儿脑脊液中的巨细胞病毒DNA。
J Infect Dis. 1994 Jun;169(6):1334-7. doi: 10.1093/infdis/169.6.1334.
2
Low correlation of human cytomegalovirus DNA amplification by polymerase chain reaction with cytomegalovirus disease in organ transplant recipients.器官移植受者中，通过聚合酶链反应进行的人巨细胞病毒DNA扩增与巨细胞病毒疾病的低相关性。
J Med Virol. 1994 Jun;43(2):187-93. doi: 10.1002/jmv.1890430217.
3
Comparison of two hybridization assays for the rapid detection of PCR amplified HSV genome sequences from cerebrospinal fluid.两种杂交检测法用于快速检测脑脊液中PCR扩增的单纯疱疹病毒基因组序列的比较。
J Virol Methods. 1994 Dec;50(1-3):175-84. doi: 10.1016/0166-0934(94)90174-0.
4
The AIDS dementia complex: II. Neuropathology.艾滋病痴呆综合征：II. 神经病理学
Ann Neurol. 1986 Jun;19(6):525-35. doi: 10.1002/ana.410190603.
5
Isolation of HTLV-III from cerebrospinal fluid and neural tissues of patients with neurologic syndromes related to the acquired immunodeficiency syndrome.从与获得性免疫缺陷综合征相关的神经综合征患者的脑脊液和神经组织中分离出人类嗜T淋巴细胞病毒III型。
N Engl J Med. 1985 Dec 12;313(24):1493-7. doi: 10.1056/NEJM198512123132401.
6
Detection of cytomegalovirus in bronchial lavage and urine using a monoclonal antibody to an HCMV early nuclear protein.使用针对人巨细胞病毒早期核蛋白的单克隆抗体检测支气管灌洗液和尿液中的巨细胞病毒。
Infection. 1988 Sep-Oct;16(5):288-92. doi: 10.1007/BF01645074.
7
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.Chelex 100作为一种从法医材料中简单提取用于基于PCR分型的DNA的介质。
Biotechniques. 1991 Apr;10(4):506-13.
8
Rapid detection of cytomegalovirus DNA in cerebrospinal fluid of AIDS patients with neurologic disorders.
J Infect Dis. 1992 Dec;166(6):1416-21. doi: 10.1093/infdis/166.6.1416.

用于快速定量检测脑脊液中人巨细胞病毒的新型聚合酶链反应检测法。

New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid.

作者信息

Vogel J U, Cinatl J, Lux A, Weber B, Driesel A J, Doerr H W

机构信息

Institute of Medical Virology, JWG-University Frankfurt, Frankfurt am Main, Germany.

出版信息

J Clin Microbiol. 1996 Feb;34(2):482-3. doi: 10.1128/jcm.34.2.482-483.1996.

DOI:10.1128/jcm.34.2.482-483.1996
PMID:8789047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228829/
Abstract

Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per microliter. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per microliter.

摘要

建立了快速Chelex提取法结合自动化杂交检测法，用于检测脑脊液中PCR扩增的人巨细胞病毒DNA。以质粒作为外标进行DNA定量。检测限为每微升10拷贝。人巨细胞病毒DNA的定量检测可在每微升10至10⁴拷贝的范围内实现。