用于快速定量检测脑脊液中人巨细胞病毒的新型聚合酶链反应检测法。
New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid.
作者信息
Vogel J U, Cinatl J, Lux A, Weber B, Driesel A J, Doerr H W
机构信息
Institute of Medical Virology, JWG-University Frankfurt, Frankfurt am Main, Germany.
出版信息
J Clin Microbiol. 1996 Feb;34(2):482-3. doi: 10.1128/jcm.34.2.482-483.1996.
Abstract
Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per microliter. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per microliter.
摘要
建立了快速Chelex提取法结合自动化杂交检测法，用于检测脑脊液中PCR扩增的人巨细胞病毒DNA。以质粒作为外标进行DNA定量。检测限为每微升10拷贝。人巨细胞病毒DNA的定量检测可在每微升10至10⁴拷贝的范围内实现。
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