采用直接逆转录聚合酶链反应法测定甲型流感病毒在禽类中的致病潜力。
Direct reverse transcriptase PCR to determine virulence potential of influenza A viruses in birds.
作者信息
Horimoto T, Kawaoka Y
机构信息
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101, USA.
出版信息
J Clin Microbiol. 1995 Mar;33(3):748-51. doi: 10.1128/jcm.33.3.748-751.1995.
Abstract
A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detected the HA gene, even in specimens that were negative for virus by standard testing in eggs. This technique, combined with sequencing of the HA cleavage site, offers a rapid and sensitive way to assess the virulence potential of avian influenza viruses. Early detection of field isolates with virulence-associated structural motifs at the HA cleavage site would allow better control of influenza among large poultry populations.
摘要
逆转录聚合酶链反应（RT-PCR）用于快速确定血凝素（HA）裂解位点序列，这是禽流感病毒毒力潜力的一个标志物。当应用于经强毒或无毒病毒实验感染的鸡的样本时，RT-PCR能一致地检测到HA基因，即使在通过标准鸡蛋检测法检测为病毒阴性的样本中也是如此。这项技术与HA裂解位点测序相结合，提供了一种快速且灵敏的方法来评估禽流感病毒的毒力潜力。早期检测到HA裂解位点带有与毒力相关结构基序的田间分离株，将有助于更好地控制大型家禽群体中的流感。
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