Mancini A, Conte B, De Marinis L, Hallgass M E, Pozza D, Oradei A, Littarru G P
Institute of Endocrinology, Catholic University, Rome.
Mol Aspects Med. 1994;15 Suppl:s249-55. doi: 10.1016/0098-2997(94)90035-3.
The levels of Coenzyme Q10 (CoQ10) were determined by HPLC in seminal fluid samples obtained from 77 patients who performed a standard semen analysis for infertility, previous phlogosis or varicocele. CoQ10 was determined in total seminal fluid (n = 60), in seminal plasma (n = 44) and in the cell pellet (n = 37). The molecule, in total fluid, showed a linear correlation with sperm count and motility. In the pellet of spermatozoa, a trend toward an inverse correlation between CoQ10 (expressed as ng/10(6) cells) and semen parameters could be observed. A different pattern was shown in varicocele patients, in whom, in total fluid, the correlation between CoQ10 and sperm count was preserved, but the one between CoQ10 and sperm motility was lacking; moreover, a higher proportion of CoQ10 was present in seminal plasma, and the inverse trend between cellular CoQ10 and sperm count and motility was not observed. These data suggest a pathophysiological role of ubiquinone in human seminal fluid and a molecular defect in the spermatozoa of varicocele patients.
通过高效液相色谱法（HPLC）测定了77例因不育、既往炎症或精索静脉曲张而进行标准精液分析的患者精液样本中辅酶Q10（CoQ10）的水平。在总精液（n = 60）、精浆（n = 44）和细胞沉淀（n = 37）中测定了CoQ10。在总液体中，该分子与精子数量和活力呈线性相关。在精子沉淀中，可以观察到CoQ10（以ng/10⁶细胞表示）与精液参数之间呈负相关趋势。精索静脉曲张患者表现出不同的模式，在他们的总液体中，CoQ10与精子数量之间的相关性得以保留，但CoQ10与精子活力之间缺乏相关性；此外，精浆中CoQ10的比例更高，并且未观察到细胞CoQ10与精子数量和活力之间的负相关趋势。这些数据表明泛醌在人类精液中具有病理生理作用，并且精索静脉曲张患者的精子存在分子缺陷。
