Springer P S, McCombie W R, Sundaresan V, Martienssen R A
Cold Spring Harbor Laboratory, NY 11724, USA.
Science. 1995 May 12;268(5212):877-80. doi: 10.1126/science.7754372.
Gene trap transposon mutagenesis can identify essential genes whose functions in later development are obscured by an early lethal phenotype. In higher plants, many genes are required for haploid gametophyte viability, so that the phenotypic effects of their disruption cannot be readily observed in the diploid plant body. The PROLIFERA (PRL) gene, identified by gene trap transposon mutagenesis in Arabidopsis, is required for megaga-metophyte and embryo development. Reporter gene expression patterns revealed that PRL was expressed in dividing cells throughout the plant. PRL is related to the MCM2-3-5 family of yeast genes that are required for the initiation of DNA replication.
基因捕获转座子诱变可鉴定出那些在后期发育中的功能被早期致死表型所掩盖的必需基因。在高等植物中，许多基因是单倍体配子体存活所必需的，因此其破坏所产生的表型效应在二倍体植物体内不易观察到。通过拟南芥中的基因捕获转座子诱变鉴定出的PROLIFERA（PRL）基因，是大配子体和胚胎发育所必需的。报告基因表达模式显示，PRL在植物的整个分裂细胞中表达。PRL与酵母基因的MCM2 - 3 - 5家族相关，该家族基因是DNA复制起始所必需的。
