胶孢炭疽菌附着胞形成过程中表达的一个基因的克隆以及该基因破坏导致的毒力显著下降。
Cloning of a gene expressed during appressorium formation by Colletotrichum gloeosporioides and a marked decrease in virulence by disruption of this gene.
作者信息
Hwang C S, Flaishman M A, Kolattukudy P E
机构信息
Ohio State Biotechnology Center, Ohio State University, Columbus 43210.
出版信息
Plant Cell. 1995 Feb;7(2):183-93. doi: 10.1105/tpc.7.2.183.
Abstract
Appressorium formation in germinating Colletotrichum gloeosporioides is induced by the surface wax of its host. One of the genes expressed uniquely in C. gloeosporioides during appressorium formation induced by the host signal has been designated cap20, and this gene and its cDNA were cloned and sequenced. Nucleotide sequences of both revealed an open reading frame that could encode a 183-amino acid polypeptide that did not have significant homology with any known proteins. Reverse transcriptase-polymerase chain reaction detected cap20 gene transcripts at the infection front on the surface and within tomato fruits infected by C. gloeosporioides. Gene-disrupted mutants incapable of expressing cap20 showed a drastically decreased virulence on avocado and tomato fruits. These results suggest that cap20 plays a significant role in the infection of the host.
摘要
胶孢炭疽菌萌发时附着胞的形成是由其寄主的表面蜡质诱导的。在寄主信号诱导的附着胞形成过程中，胶孢炭疽菌中独特表达的一个基因被命名为cap20，该基因及其cDNA被克隆并测序。两者的核苷酸序列均显示出一个开放阅读框，可编码一个183个氨基酸的多肽，该多肽与任何已知蛋白质均无显著同源性。逆转录聚合酶链反应在胶孢炭疽菌感染的番茄果实表面和内部的感染前沿检测到cap20基因转录本。无法表达cap20的基因破坏突变体在鳄梨和番茄果实上的毒力大幅下降。这些结果表明cap20在寄主感染中起重要作用。
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