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使用高效液相色谱-紫外-可见光电二极管阵列检测-质谱法测定番红花(藏红花,Crocus sativus L.)粗提物中的成分

Determination of saffron (Crocus sativus L.) components in crude plant extract using high-performance liquid chromatography-UV-visible photodiode-array detection-mass spectrometry.

作者信息

Tarantilis P A, Tsoupras G, Polissiou M

机构信息

Department of General Sciences, Agricultural University of Athens, Greece.

出版信息

J Chromatogr A. 1995 May 5;699(1-2):107-18. doi: 10.1016/0021-9673(95)00044-n.

DOI:10.1016/0021-9673(95)00044-n
PMID:7757208
Abstract

The determination of saffron components in crude plant extracts by high-performance liquid chromatography-UV-visible photodiode-array detection on-line with mass spectrometry is described. The method is shown to be suitable for the determination of picrocrocin, the glycosidic precursor of safranal, safranal and flavonoids; it is the technique of choice for the analysis of crocetin glycosides (crocins) carrying one up to five glucoses and differentiation of their trans and cis isomers.

摘要

描述了通过高效液相色谱-紫外-可见光电二极管阵列检测与质谱联用在线测定植物粗提物中藏红花成分的方法。该方法适用于测定藏红花醛的糖苷前体苦藏花素、藏红花醛和黄酮类化合物;它是分析带有一至五个葡萄糖的藏红花酸糖苷(藏花素)及其反式和顺式异构体的首选技术。

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