Anaissie E, Samonis G, Kontoyiannis D, Costerton J, Sabharwal U, Bodey G, Raad I
Department of Medical Specialties, University of Texas, M.D. Anderson Cancer Center, Houston 77030, USA.
Eur J Clin Microbiol Infect Dis. 1995 Feb;14(2):134-7. doi: 10.1007/BF02111873.
Adult cancer patients were prospectively studied to determine the relationship between ultrastructural and microbiologic catheter colonization and clinical catheter-related infections. Participants included 38 patients whose central venous catheters were removed because of suspected catheter infection (16 patients) or other noninfectious causes (22 controls). The presence of clinical infection was determined. Catheters were examined by microbiologic methods (sonication and roll-plate culture) and by scanning and transmission electron microscopy. Ultrastructural microbial colonization and biofilm formation were universal and occurred as early as one day after catheter insertion. The extent of biofilm formation was unrelated to the clinical status of patient or the catheter microbiological findings. Secondary seeding of catheters was rarely seen. Catheter microbial biofilm formation occurs early, is universal and does not necessarily represent an infectious condition.
对成年癌症患者进行前瞻性研究，以确定超微结构与微生物导管定植以及临床导管相关感染之间的关系。参与者包括38名患者，其中16名患者因疑似导管感染而拔除中心静脉导管，22名患者因其他非感染性原因拔除导管（作为对照）。确定是否存在临床感染。通过微生物学方法（超声处理和滚板培养）以及扫描和透射电子显微镜对导管进行检查。超微结构微生物定植和生物膜形成普遍存在，且早在导管插入后一天就出现。生物膜形成的程度与患者的临床状况或导管微生物学检查结果无关。很少见到导管的二次定植。导管微生物生物膜形成出现早、普遍存在，且不一定代表感染状态。
