Braginskaia P S, Tikhonova A S, Chaĭkovskaia S M
Antibiotiki. 1976 Feb;21(2):118-22.
Efficiency of 3 methods for testing sterility of antibiotic ophthalmic ointments was compared. During storage of antibiotic ophthalmic ointments infected with bacteria some "self-sterilization" takes place mainly due to death of the antibiotic sensitive bacteria. The results of determination of the ointment non-sterility depends on the level of their bacterial loading, sensitivity of the cultures to the antibiotic and time of the culture contact with the antibiotic in the ointment before its testing. The method of the ointment solution in isopropylmiristate filtration through a membrane filter followed by washing of the filter from the antibiotic and its placing into a thioglycol medium proved to be most effective for determination of bacterial admixtures in antibiotic ointments.
比较了3种检测抗生素眼膏无菌性的方法。在储存被细菌感染的抗生素眼膏期间，主要由于对抗生素敏感的细菌死亡，会发生一些“自我灭菌”现象。眼膏非无菌性的测定结果取决于其细菌负载水平、培养物对抗生素的敏感性以及在检测前培养物与眼膏中抗生素接触的时间。通过膜过滤器过滤异丙基肉豆蔻酸酯中的眼膏溶液，然后从抗生素中冲洗过滤器并将其置于硫乙醇酸盐培养基中的方法，被证明是测定抗生素眼膏中细菌杂质最有效的方法。
