Galons J P, Job C, Gillies R J
Department of Biochemistry, College of Medicine, Arizona Health Sciences Center, Tucson 85724, USA.
Magn Reson Med. 1995 Mar;33(3):422-6. doi: 10.1002/mrm.1910330317.
The purpose of this study was to study the metabolic events during a slow acidosis in three different cell lines by combining 31P magnetic resonance spectroscopy and hollow fiber bioreactor technology. The rate of change in intracellular pH, glycerophosphorylcholine (GPC), phophorylcholine (PCho), and nucleoside-triphosphate (NTP) levels were measured during 8 h of acidosis and 16 h of recovery in EPO, EAT, and RN1a cells, three cultured mammalians cell lines. Our results show a significant increase in GPC levels to 330 +/- 21.540 +/- 25, and 220 +/- 21% of their initial value correlated to a decrease of PCho levels to 57 +/- 14.58 +/- 17 and 45 +/- 15% of their initial value in EAT, RN1a, and EPO cells, respectively. These changes are discussed in terms of perturbation of energetic metabolism in cells undergoing a slow acidosis.
本研究的目的是通过结合³¹P磁共振波谱和中空纤维生物反应器技术，研究三种不同细胞系在缓慢酸中毒过程中的代谢事件。在EPO、EAT和RN1a细胞（三种培养的哺乳动物细胞系）酸中毒8小时和恢复16小时期间，测量细胞内pH值、甘油磷酸胆碱（GPC）、磷酸胆碱（PCho）和核苷三磷酸（NTP）水平的变化率。我们的结果显示，在EAT、RN1a和EPO细胞中，GPC水平分别显著增加至其初始值的330±21、540±25和220±21%，这与PCho水平分别降至其初始值的57±14、58±/17和45±15%相关。这些变化从经历缓慢酸中毒的细胞中能量代谢的扰动方面进行了讨论。
