人体乳腺肿瘤的体内和体外31P核磁共振光谱比较：磷脂代谢的变化
A comparison of in vivo and in vitro 31P NMR spectra from human breast tumours: variations in phospholipid metabolism.
作者信息
Smith T A, Glaholm J, Leach M O, Machin L, Collins D J, Payne G S, McCready V R
机构信息
Department of Nuclear Medicine, Royal Marsden Hospital, Sutton, Surrey, UK.
出版信息
Br J Cancer. 1991 Apr;63(4):514-6. doi: 10.1038/bjc.1991.122.
Abstract
An in vivo 31P NMR spectrum was obtained from each of four human breast tumours. The phosphomonoester and phosphodiester region of each spectrum consisted of a broad peak. Chemical extracts from samples of each of the tumours obtained at resection were examined on a high field strength NMR system. The phosphomonoester region in the spectrum from each extract resolved into three peaks consisting of phosphocholine, phosphoethanolamine and a nucleoside monophosphate. The phosphodiester region resolved into two components, glycerophosphorylcholine and glycerophosphorylethanolamine. Comparing the in vivo and in vitro data from each tumour showed that the contribution of phosphodiester was much lower in the in vitro spectra. We believe this to be a consequence of phospholipid, which would not appear in the aqueous extract, contributing to the phosphodiester peak in vivo.
摘要
从四个人类乳腺肿瘤中分别获取了体内31P NMR谱。每个谱的磷酸单酯和磷酸二酯区域均由一个宽峰组成。对手术切除时获取的每个肿瘤样本的化学提取物在高场强NMR系统上进行了检测。每个提取物谱中的磷酸单酯区域解析为三个峰，分别由磷酸胆碱、磷酸乙醇胺和核苷单磷酸组成。磷酸二酯区域解析为两个成分，甘油磷酰胆碱和甘油磷酰乙醇胺。比较每个肿瘤的体内和体外数据表明，体外谱中磷酸二酯的贡献要低得多。我们认为这是由于磷脂（其不会出现在水提取物中）在体内对磷酸二酯峰有贡献的结果。
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