Luo S Q, Lin L Z, Cordell G A
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago.
Phytochemistry. 1993 Jul;33(5):1197-1205. doi: 10.1016/0031-9422(93)85049-w.
From the roots of Bupleurum wenchuanense 14 derivatives of saikosaponin were isolated and identified as 2"-O-beta-D-xylopyranosylsaikosaponin b2, 3",6"-O O-diacetylsaikosaponin b2, 2"-O-beta-D-glucopyranosylsaikosaponin b2, saikosaponin b2, 6"-O-acetylsaikosaponin b2, saikosaponin d, 2"-O-acetylsaikosaponin d, 3"-O-acetylsaikosaponin d,6"-O-acetylsaikosaponin d, 16-epichikusaikoside, prosaikogenin G, saikosaponin a,2"-O-acetylsaikosaponin a and 3"-O-acetylsaikosaponin a. The first two compounds are new derivatives of saikosaponin and this is the first isolation of prosaikogenin G from a plant. Their complete 1H and 13C NMR assignments were made by using a combination of 2D NMR techniques (DQF-COSY, HOHAHA, ROESY, HETCOR, HMQC and HMBC). Some of the compounds showed cytotoxic activity against the P-388 cell line.
从汶川柴胡的根部分离并鉴定出14种柴胡皂苷衍生物，分别为2”-O-β-D-吡喃木糖基柴胡皂苷b2、3”,6”-O,O-二乙酰基柴胡皂苷b2、2”-O-β-D-吡喃葡萄糖基柴胡皂苷b2、柴胡皂苷b2、6”-O-乙酰基柴胡皂苷b2、柴胡皂苷d、2”-O-乙酰基柴胡皂苷d、3”-O-乙酰基柴胡皂苷d、6”-O-乙酰基柴胡皂苷d、16-表赤库皂苷、原柴胡皂苷元G、柴胡皂苷a、2”-O-乙酰基柴胡皂苷a和3”-O-乙酰基柴胡皂苷a。前两种化合物是柴胡皂苷的新衍生物，这是首次从植物中分离得到原柴胡皂苷元G。通过二维核磁共振技术（DQF-COSY、HOHAHA、ROESY、HETCOR、HMQC和HMBC）的组合确定了它们完整的1H和13C核磁共振归属。其中一些化合物对P-388细胞系显示出细胞毒性活性。
