Weigl B H, Holobar A, Trettnak W, Klimant I, Kraus H, O'Leary P, Wolfbeis O S
Joanneum Research, Institute for Sensor Interfaces, Graz, Austria.
J Biotechnol. 1994 Feb 14;32(2):127-38. doi: 10.1016/0168-1656(94)90175-9.
A triple sensor unit consisting of opto-chemical sensors for measurement of pH, oxygen and carbon dioxide in bioreactors is presented. The pH and the CO2 sensor are based on the color change of a pH-sensitive dye immobilized on a polymeric support. The resulting changes in absorption are monitored through optical fibers. The oxygen sensor is based on the quenching of the fluorescence of a metal-organic dye. All three sensors are fully LED compatible. The sensitive membranes consist of plastic films and can be stored and replaced conveniently. The sensors are sterilizable with hydrogen peroxide and ethanol. In addition, the pH sensor is steam sterilizable. Accuracy, resolution and reproducibility fulfill the requirements for use in biotechnological applications. Calibration procedures for each sensor are presented. The working principle and the performance of all three sensors are described, with particular emphasis given to their application in bioreactors.
本文介绍了一种用于测量生物反应器中pH值、氧气和二氧化碳的三传感器单元，该单元由光化学传感器组成。pH传感器和二氧化碳传感器基于固定在聚合物载体上的pH敏感染料的颜色变化。通过光纤监测由此产生的吸收变化。氧气传感器基于金属有机染料荧光的猝灭。所有三个传感器均完全兼容LED。敏感膜由塑料薄膜组成，便于储存和更换。这些传感器可用过氧化氢和乙醇进行消毒。此外，pH传感器可进行蒸汽消毒。其准确性、分辨率和可重复性满足生物技术应用的要求。文中给出了每个传感器的校准程序。描述了所有三个传感器的工作原理和性能，特别强调了它们在生物反应器中的应用。
