Swinkels B W, van Ooyen A J, Bonekamp F J
Gist-Brocades B.V., Research and Development, Delft, The Netherlands.
Antonie Van Leeuwenhoek. 1993;64(2):187-201. doi: 10.1007/BF00873027.
Several different yeast species have been developed into systems for efficient heterologous gene expression. In this paper we review foreign gene expression in the dairy yeast Kluyveromyces lactis. This yeast presents several advantageous properties in comparison to other yeast species. These include its impressive secretory capacities, its excellent fermentation characteristics on large scale, its food grade status and the availability of both episomal and integrative expression vectors. Moreover, in contrast to the methylotrophic yeasts that are frequently used for the expression of foreign genes, K. lactis does not require explosion-proof fermentation equipment. Here, we present an overview of the available tools for heterologous gene expression in K. lactis (available promoters, vector systems, etc). Also, the production of prochymosin, human serum albumin and pancreatic phospholipase by K. lactis is discussed in more detail, and used to rate the achievements of K. lactis with respect to other micro-organisms in which these proteins have been produced.
几种不同的酵母物种已被开发成用于高效异源基因表达的系统。在本文中，我们综述了外源基因在产奶酵母乳酸克鲁维酵母中的表达。与其他酵母物种相比，这种酵母具有几个优势特性。这些特性包括其令人印象深刻的分泌能力、大规模发酵的优良特性、食品级地位以及附加型和整合型表达载体的可用性。此外，与常用于外源基因表达的甲基营养型酵母不同，乳酸克鲁维酵母不需要防爆发酵设备。在此，我们概述了乳酸克鲁维酵母中用于异源基因表达的可用工具（可用的启动子、载体系统等）。此外，还更详细地讨论了乳酸克鲁维酵母生产凝乳酶原、人血清白蛋白和胰磷脂酶的情况，并以此来评估乳酸克鲁维酵母相对于生产这些蛋白质的其他微生物所取得的成果。
