Li S Y, Ramsden J J, Prenosil J E, Heinzle E
Department of Chemical Engineering, ETH, Zürich, Switzerland.
Biotechnol Prog. 1994 Sep-Oct;10(5):520-4. doi: 10.1021/bp00029a010.
A novel optical method was used for quantitative characterization and continuous measurement of both the adhesion and spreading of mammalian cells on inorganic surfaces. It is based upon the effective refractive index change caused by cells when they adhere to a planar optical waveguide. We have applied this technique to measure the kinetics of the adhesion and spreading processes of baby hamster kidney (BHK) cells adhering to surfaces coated with fibronectin and under different culture conditions (PBS, medium, serum, EDTA). In comparison, hybridoma cells are only adsorbed to the surface and do not spread at all. Moreover, this technique also allows the mass of an adsorbed protein adlayer to be determined very precisely and thus provides a valuable tool for screening suitable substrata as well as determining the influence of different culture conditions on cell adhesion and spreading. This sensitive test for substrate influence could be important in toxicity tests using adherent animal cells.
一种新颖的光学方法被用于对哺乳动物细胞在无机表面上的黏附与铺展进行定量表征和连续测量。它基于细胞黏附到平面光波导时所引起的有效折射率变化。我们已应用该技术来测量幼仓鼠肾（BHK）细胞在涂有纤连蛋白的表面上以及在不同培养条件（PBS、培养基、血清、EDTA）下黏附与铺展过程的动力学。相比之下，杂交瘤细胞仅吸附在表面，根本不铺展。此外，该技术还能非常精确地测定吸附蛋白层的质量，从而为筛选合适的基质以及确定不同培养条件对细胞黏附与铺展的影响提供了一个有价值的工具。这种对基质影响的灵敏测试在使用贴壁动物细胞的毒性测试中可能很重要。
