Shen B Q, Greenfield P F, Reid S
Department of Chemical Engineering, University of Queensland, St. Lucia, Australia.
Cytotechnology. 1994;16(1):51-8. doi: 10.1007/BF00761779.
A composite gel system has been developed combining the chemical and physical properties of calcium alginate and agarose gels. The results of growing composite gel immobilized hybridoma SPO1 cells in a protein-free medium within a fluidized-bed perfusion bioreactor are presented in this paper. During the continuous operation of this system, the total cell density reached 3.9 x 10(7) cells per ml of beads (viability 79.6%). The specific productivity of monoclonal antibody of the immobilized hybridoma cells reached more than 1.5 micrograms per 10(6) viable cells per hour, compared with 0.5 for non-immobilized viable cells grown in a one liter agitated bioreactor with the same medium. Significant increases in cell metabolic activities, including substrate utilization and byproduct formation, were also observed. Leaching of materials from the beads was evident and the major fraction of released materials was alginate.
一种结合了海藻酸钙凝胶和琼脂糖凝胶化学与物理性质的复合凝胶系统已被研发出来。本文展示了在流化床灌注生物反应器中，在无蛋白培养基中培养复合凝胶固定化杂交瘤SPO1细胞的结果。在该系统的连续运行过程中，每毫升珠子中的总细胞密度达到3.9×10⁷个细胞（活力为79.6%）。与在相同培养基的一升搅拌式生物反应器中培养的未固定化活细胞的0.5相比，固定化杂交瘤细胞的单克隆抗体比生产率达到每10⁶个活细胞每小时超过1.5微克。还观察到细胞代谢活动显著增加，包括底物利用和副产物形成。珠子中有明显的物质浸出，释放物质的主要部分是海藻酸盐。
