Matsumoto S, Ikura K, Ueda M, Sasaki R
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Plant Mol Biol. 1995 Mar;27(6):1163-72. doi: 10.1007/BF00020889.
Erythropoietin (Epo), a glycoprotein that regulates the formation of erythrocytes in mammals, was produced in cultured tobacco BY2 cells (Nicotiana tabacum L. cv. Bright Yellow 2) by introducing human Epo cDNA via Agrobacterium tumefaciens-mediated gene transfer. Epo was correctly processed and subsequently penetrated the plasma membrane of tobacco cells. However, it remained attached to the cell wall and was not released into the culture medium. Although Epo produced by tobacco cells was glycosylated with N-linked oligosaccharides, these carbohydrates were smaller than those of the recombinant Epo produced in mammalian cells. Epo produced in tobacco exhibited in vitro biological activities by inducing the differentiation and proliferation of erythroid cells. However, it had no in vivo biological activities. A lectin-binding assay indicated the lack of sialic acid residues in the N-linked oligosaccharides of Epo, suggesting that Epo was removed from the circulation before it reached erythropoietic tissues.
促红细胞生成素（Epo）是一种调节哺乳动物红细胞形成的糖蛋白，通过根癌农杆菌介导的基因转移引入人Epo cDNA，在培养的烟草BY2细胞（烟草品种亮黄2）中产生。Epo经过正确加工，随后穿透烟草细胞的质膜。然而，它仍附着在细胞壁上，未释放到培养基中。尽管烟草细胞产生的Epo用N - 连接寡糖进行了糖基化，但这些碳水化合物比在哺乳动物细胞中产生的重组Epo的碳水化合物小。烟草中产生的Epo通过诱导红系细胞的分化和增殖表现出体外生物学活性。然而，它没有体内生物学活性。凝集素结合试验表明Epo的N - 连接寡糖中缺乏唾液酸残基，这表明Epo在到达造血组织之前就从循环中被清除了。
