Meade C J, Mertin J
Int Arch Allergy Appl Immunol. 1976;51(1):2-24. doi: 10.1159/000231575.
Subcutaneous injection with certain polyunsaturated fatty acids (PUFA), in particular linoleic acid (C18:2), has recently been shown to prolong the survival of skin allografts in mice and to reduce both primary and secondary cytotoxic responses by isolated spleen cells. In this study we have examined changes in the lymphoid and reticulo-endothelial systems of both grafted and ungrafted mice treated according to the schedules shown to prolong allograft survival. Many of the changes in the lymphoid and reticulo-endothelial systems which can be produced by allografting could also be produced in ungrafted animals by PUFA treatment. These changes included: increased 125IudR uptake by spleen, lymph nodes, and bone marrow, increased spleen and lymph node weight, increased proportion of red pulp and granulocyte precursors in the spleen, and reticulo-endothelial activation as shown by an increased rate of carbon clearance. The combined effects of allografting and C18:2 on peripheral lymph node weight were greater than the effect of either treatment alone, but fell short of the sum of both treatments. Allografting increased 125IudR uptake/unit organ weight, not only in the peripheral lymph nodes, but also in bone marrow, thymus and spleen. When allografted animals were C18:2 treated, this increase was considerably reduced, uptake being little different from that in C18:2 treated ungrafted controls. The number of theta-positive cells in the spleen was markedly increased by a tumor allograft, but such an increase could not be seen in allografted animals when treated with C18:2. Whilst prolonged C18:2 treatment led to destructive changes in the spleen, immunoinhibition could still be demonstrated after shorter treatments with C18:2 when no gross histological evidence for tissue destruction was apparent. Partial reversibility of inhibition of cytotoxic activity of isolated spleen cells could be domonstrated when treatment was discontinued.
最近研究表明，皮下注射某些多不饱和脂肪酸（PUFA），特别是亚油酸（C18:2），可延长小鼠皮肤同种异体移植物的存活时间，并降低分离的脾细胞的初次和二次细胞毒性反应。在本研究中，我们根据已证明能延长同种异体移植物存活时间的方案，对移植和未移植小鼠的淋巴和网状内皮系统的变化进行了研究。同种异体移植所引起的淋巴和网状内皮系统的许多变化，也可通过PUFA处理在未移植的动物中产生。这些变化包括：脾脏、淋巴结和骨髓对125IudR的摄取增加，脾脏和淋巴结重量增加，脾脏中红髓和粒细胞前体的比例增加，以及碳清除率增加所显示的网状内皮系统激活。同种异体移植和C18:2对外周淋巴结重量的联合作用大于单独任何一种处理的作用，但低于两种处理作用之和。同种异体移植不仅增加了外周淋巴结单位器官重量的125IudR摄取，也增加了骨髓、胸腺和脾脏的摄取。当对同种异体移植动物进行C18:2处理时，这种增加显著减少，摄取量与C18:2处理的未移植对照动物几乎没有差异。肿瘤同种异体移植使脾脏中θ阳性细胞的数量显著增加，但在用C18:2处理的同种异体移植动物中未见这种增加。虽然长期C18:2处理会导致脾脏出现破坏性变化，但在较短时间的C18:2处理后，当没有明显的组织破坏的大体组织学证据时，仍可证明存在免疫抑制。当停止处理时，可证明分离的脾细胞的细胞毒性活性抑制具有部分可逆性。
