超氧化物歧化酶可保护大肠杆菌免受需氧热休克的影响。
Superoxide dismutase protects against aerobic heat shock in Escherichia coli.
作者信息
Benov L, Fridovich I
机构信息
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA.
出版信息
J Bacteriol. 1995 Jun;177(11):3344-6. doi: 10.1128/jb.177.11.3344-3346.1995.
Abstract
Exposure of a superoxide dismutase-null (sodA sodB) strain of Escherichia coli to aerobic heat stress (45 to 48 degrees C) caused a profound loss of viability, whereas the same heat stress applied anaerobically had a negligible effect. A superoxide dismutase-competent parental strain was resistant to the lethal effect of the aerobic heating. It follows that aerobic heating imposes an oxidative burden of which O2- must be a major component. This effect is not seen at 53 degrees C, presumably because, at this higher temperature, direct thermolability of vital cell components overrides the effect of superoxide radicals.
摘要
将大肠杆菌的超氧化物歧化酶缺失（sodA sodB）菌株暴露于需氧热应激（45至48摄氏度）会导致其活力大幅丧失，而在厌氧条件下施加相同的热应激则影响可忽略不计。具有超氧化物歧化酶活性的亲本菌株对需氧加热的致死效应具有抗性。由此可见，需氧加热会施加氧化负担，其中O2-必定是主要成分。在53摄氏度时未观察到这种效应，大概是因为在这个更高的温度下，重要细胞成分的直接热不稳定性超过了超氧自由基的影响。
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