Dozortsev D, Rybouchkin A, De Sutter P, Qian C, Dhont M
Department of Gynaecology and Obstetrics, University Hospital of Gent, Belgium.
Hum Reprod. 1995 Feb;10(2):403-7. doi: 10.1093/oxfordjournals.humrep.a135952.
The aim of this study was to investigate whether the human spermatozoon participates in the activation of human oocytes following intracytoplasmic sperm injection (ICSI) and if so, by what mechanism. In the first series of experiments, we randomized human oocytes which had remained unfertilized after in-vitro fertilization (IVF) or ICSI, for intracytoplasmic injection with live spermatozoa, spermatozoa presumed to be dead and no spermatozoa. Secondly, unfertilized human oocytes and freshly ovulated mouse oocytes were randomized for intracytoplasmic and sub-zonal injection with human sperm cytosolic fraction (CF) before and after heat treatment. We found that oocyte injection with initially motile spermatozoa induces human oocyte activation at a significantly higher rate than injection with dead spermatozoa (61 versus 0%; P < 0.001) or injection without a spermatozoon (61 versus 14%; P < 0.001). Intracytoplasmic injection of CF activated both human and mouse oocytes at the same rate as sperm injection of human oocytes (activation rates of 70 and 65% respectively). This effect was greatly reduced by heat treatment of the CF. From these experiments we conclude firstly that the human spermatozoon injected intracytoplasmically contributes to human oocyte activation and secondly that the spermatozoon releases into the oocyte a heat-sensitive, intracellularly active factor, which is not species-specific.
本研究的目的是调查在卵胞浆内单精子注射（ICSI）后人类精子是否参与人类卵母细胞的激活，如果参与，其机制是什么。在第一组实验中，我们将体外受精（IVF）或ICSI后仍未受精的人类卵母细胞随机分为三组，分别进行胞浆内注射活精子、假定死亡的精子和不注射精子。其次，将未受精的人类卵母细胞和刚排卵的小鼠卵母细胞随机分为两组，分别在热处理前后进行胞浆内和卵周隙注射人类精子胞质成分（CF）。我们发现，向卵母细胞注射最初有活力的精子诱导人类卵母细胞激活的比率显著高于注射死亡精子（61%对0%；P<0.001）或不注射精子（61%对14%；P<0.001）。向人类卵母细胞胞浆内注射CF激活人类和小鼠卵母细胞的比率与向人类卵母细胞注射精子相同（激活率分别为70%和65%）。CF经热处理后，这种作用大大降低。从这些实验中我们首先得出结论，胞浆内注射的人类精子有助于人类卵母细胞的激活，其次得出结论，精子向卵母细胞释放一种热敏感的、细胞内活性因子，该因子无物种特异性。
