Riser E, Noone P, Bonnet M L
J Clin Pathol. 1976 Apr;29(4):305-8. doi: 10.1136/jcp.29.4.305.
A new indirect fluorescent typing method for Klebsiella species is compared with an established method, capsular swelling. The fluorescent antibody (FA) technique was tested with standards and unknowns, and the results were checked by capsular swelling. Several unknowns were sent away for confirmation of typing, by capsular swelling. The FA method was also tried by a technician in the routine department for blind identification of standards. Fluorescence typing gives close correlation with the established capsular swelling technique but has greater sensitivity; allows more econimical use of expensive antisera; possesses greater objectivity as it requires less operator skill in the reading of results; resolves most of the cross reactions observed with capsular swelling; and has a higher per cent success rate in identification.
一种用于克雷伯菌属的新型间接荧光分型方法与一种既定方法——荚膜肿胀试验进行了比较。荧光抗体（FA）技术用标准菌株和未知菌株进行了测试，结果通过荚膜肿胀试验进行核对。若干未知菌株被送去通过荚膜肿胀试验进行分型确认。常规科室的一名技术人员也尝试使用FA方法对标准菌株进行盲法鉴定。荧光分型与既定的荚膜肿胀技术密切相关，但具有更高的灵敏度；能更经济地使用昂贵的抗血清；由于在结果判读中所需的操作人员技能较少，因而具有更高的客观性；解决了大多数在荚膜肿胀试验中观察到的交叉反应；并且在鉴定中具有更高的成功率。
