Willingham M C, Pastan I
Cell. 1978 Mar;13(3):501-7. doi: 10.1016/0092-8674(78)90323-9.
A highly sensitive television camera (silicon intensifier target) has been combined with fluorescence microscopy to examine living cultured cells. This system is termed Video Intensification Microscopy (VIM). By using very small amounts of excitation light, one limits the damage to living cells from excessive illumination and is able to visualize fluorescence probes for periods up to 24 hr without bleaching. With VIM, the cellular uptake and fate of two rhodamine-labeled proteins, concanavalin A and alpha2 macroglobulin, have been followed for up to 24 hr. These proteins were first located in endocytic vesicles with a low phase density. Later, at 24 hr, alpha2 macroglobulin was located in phase-dense structures, probably secondary lysosomes. Both the fluorescent endocytic vesicles and lysosomes were observed to undergo saltatory motion. VIM combined with fluorescence promises to have a widespread application in the study of the behavior of living cells.
一台高灵敏度电视摄像机（硅增强靶）已与荧光显微镜相结合，用于检测活的培养细胞。该系统被称为视频增强显微镜（VIM）。通过使用极少量的激发光，可限制过度照明对活细胞的损伤，并能够在长达24小时内观察荧光探针而不发生漂白。利用VIM，已对两种罗丹明标记的蛋白质——伴刀豆球蛋白A和α2巨球蛋白在细胞内的摄取和命运进行了长达24小时的追踪。这些蛋白质最初位于具有低相密度的内吞小泡中。后来，在24小时时，α2巨球蛋白位于相致密结构中，可能是次级溶酶体。观察到荧光内吞小泡和溶酶体都进行跳跃运动。VIM与荧光相结合有望在活细胞行为研究中得到广泛应用。
