Grieder N C, Nellen D, Burke R, Basler K, Affolter M
Biozentrum, Universität Basel, Switzerland.
Cell. 1995 Jun 2;81(5):791-800. doi: 10.1016/0092-8674(95)90540-5.
Cytokines of the TGF beta superfamily regulate many aspects of cellular function by activating receptor complexes consisting of two distantly related serine/threonine kinases. Previous studies have indicated that Drosophila dpp uses similar signaling complexes and strictly requires the punt and thick veins receptors to transduce the signal across the membrane. Here, we show that the schnurri (shn) gene is required for many aspects of dpp signaling. Genetic epistasis experiments indicate that shn functions downstream of the dpp signal and its receptors. The shn gene encodes a large protein similar to a family of mammalian zinc finger transcription factors. The shn protein might therefore act as a nuclear target in the dpp signaling pathway directly regulating the expression of dpp-responsive genes.
转化生长因子β超家族的细胞因子通过激活由两个远亲丝氨酸/苏氨酸激酶组成的受体复合物来调节细胞功能的许多方面。先前的研究表明，果蝇的dpp使用类似的信号复合物，并且严格需要punt和thick veins受体来跨膜转导信号。在这里，我们表明schnurri（shn）基因对于dpp信号传导的许多方面都是必需的。遗传上位性实验表明，shn在dpp信号及其受体的下游起作用。shn基因编码一种与哺乳动物锌指转录因子家族相似的大蛋白。因此，shn蛋白可能作为dpp信号通路中的核靶点，直接调节dpp反应基因的表达。
