Zhang Y Y, Lok A S, Chan D T, Widell A
Department of Medical Microbiology, University of Lund, Malmö University Hospital, Sweden.
J Clin Microbiol. 1995 Nov;33(11):2931-4. doi: 10.1128/jcm.33.11.2931-2934.1995.
A hepatitis C virus (HCV) genotyping PCR assay based on type-specific primers was expanded to include genotype 6a as well as genotypes 1a, 1b, 2a, and 3a. The nucleotide sequences of a 194-bp fragment in the center of the HCV core gene showed that the homologies between genotype 6a and genotypes 1a, 1b, 2a, 2b, 3a, and 5 were 81.2, 82.1, 73.8, 77.3, 81.4, and 78.9%, respectively. A high degree of homology (99.6%) was seen in the amplified core region among eight clinically unrelated genotype 6a isolates. Although the Hong Kong Chinese patients had predominantly genotype 1b (70%), it was noteworthy that genotype 6a was the second most common genotype (14%). Four other HCV genotypes--1a, 1b, 2a, and 2b--were also present. In contrast, HCV infection by mainland China was confined to genotypes 1b and 2a. Thus, we found a greater diversity of HCV genotypes in Hong Kong than in mainland China.
一种基于型特异性引物的丙型肝炎病毒（HCV）基因分型聚合酶链反应（PCR）检测方法得到了扩展，纳入了6a型以及1a、1b、2a和3a型。HCV核心基因中部一个194bp片段的核苷酸序列显示，6a型与1a、1b、2a、2b、3a和5型之间的同源性分别为81.2%、82.1%、73.8%、77.3%、81.4%和78.9%。在8株临床无关的6a型分离株的扩增核心区域观察到高度同源性（99.6%）。虽然香港华人患者中主要是1b型（70%），但值得注意的是，6a型是第二常见的基因型（14%）。还存在其他四种HCV基因型——1a、1b、2a和2b。相比之下，中国大陆的HCV感染局限于1b型和2a型。因此，我们发现香港的HCV基因型比中国大陆更多样化。
