van Wissen B, Wolf J P, Bomsel-Helmreich O, Frydman R, Jouannet P
Laboratoire de Biologie de la Reproduction, Hôpital Cochin, Paris, France.
Hum Reprod. 1995 Mar;10(3):642-8. doi: 10.1093/oxfordjournals.humrep.a136003.
The sequential transformations of human sperm nuclei in human eggs after subzonal insemination (SUZI; n = 104) and the influence of sperm defects on this timing were studied. This chronology was compared to that of two control series of zygotes obtained after SUZI with normal spermatozoa (n = 35) and after in-vitro fertilization (IVF) with normal donor spermatozoa (D-IVF; n = 220). Pronuclear formation took place between 4.5 and 10.5 h post-SUZI for 92.8% of the zygotes. They remained visible for 13 h and began to disappear 18.5 h post-SUZI. The timespan 3 h. Zygotes obtained after D-IVF had a similar rate of pronuclear disappearance but approximately 4 h later. The second cell cycle was more rapid for zygotes obtained by D-IVF than by SUZI, but the developmental rate of zygotes obtained by SUZI varied according to sperm phenotypes. For patients with previous unexplained IVF failures (control group with normal spermatozoa), the developmental rate was lower, suggesting the influence of oocyte quality. In conclusion, the end of the first cell cycle of zygotes obtained by insemination under the zona pellucida appears 4 h earlier compared to zygotes obtained after insemination outside the zona pellucida.
研究了人类卵母细胞经透明带下授精（SUZI；n = 104）后人类精核的连续变化，以及精子缺陷对这一进程时间的影响。将这一进程时间与两个对照组进行比较，一组是经SUZI使用正常精子获得的受精卵（n = 35），另一组是经体外受精（IVF）使用正常供体精子获得的受精卵（D-IVF；n = 220）。92.8%的受精卵在SUZI后4.5至10.5小时形成原核。原核在SUZI后13小时内可见，并在SUZI后18.5小时开始消失。时间跨度为3小时。D-IVF后获得的受精卵原核消失率相似，但大约晚4小时。D-IVF获得的受精卵的第二个细胞周期比SUZI获得的受精卵更快，但SUZI获得的受精卵的发育速度因精子表型而异。对于既往有不明原因IVF失败的患者（精子正常的对照组），发育速度较低，提示卵母细胞质量的影响。总之，与在透明带外授精后获得的受精卵相比，在透明带下授精获得的受精卵的第一个细胞周期结束时间提前4小时。
