Johansson M E, Bergquist N R, Grandien M
J Immunol Methods. 1976;11(3-4):265-72. doi: 10.1016/0022-1759(76)90119-8.
One potentially important cause of disturbing staining of cell cultures used for immunofluorescence (IF) tests was found to be a specific reaction between calf serum and corresponding antibodies. A simple method utilizing polymerized calf serum as an immunosorbent was used for the removal of the antibodies causing the undesired reactions. One to three cycles of absorption with the calf serum immunosorbent were highly efficient in removing antibodies to calf serum, whereas the titer of a heterologous antiserum (anti-egg albumin) was only slightly affected by the same procedure. An enzyme linked immunosorbent assay (ELISA) was used for determination of the efficacy of the absorption method.
免疫荧光（IF）检测所用细胞培养物出现令人困扰的染色现象，一个潜在的重要原因是小牛血清与相应抗体之间的特异性反应。一种利用聚合小牛血清作为免疫吸附剂的简单方法被用于去除引起不良反应的抗体。用小牛血清免疫吸附剂进行一到三个循环的吸附，能高效去除针对小牛血清的抗体，而相同操作对异种抗血清（抗卵清蛋白）的效价影响甚微。采用酶联免疫吸附测定（ELISA）来确定吸附方法的效果。
